Abstract: Objective To establish a GFP-labelled radioresistant xenograft tumor model of human nasopharyngeal carcinoma（NPC） and build the foundation for further study. Methods Lentivirus was used to establish a stable， radioresistant human NPC cell line expressing GFP（GFP-CNE-2R） and a corresponding non-radioresistant cell line （GFP-CNE-2）.Both lines were subcutaneously implanted into BALB/c-nu nude mice；when the tumors had reached 1 cm in size，the animals were irradiated with 10 Gy in 1 fraction.Tumor proliferation and metastasis were analyzed in vivo using dynamic imaging technology. Results GFP-CNE-2R and GFP-CNE-2 lines were successfully established， and clone formation assays showed GFP-CNE-2R cells to be radioresistant.Xenograft tumors were grown from GFP-CNE-2R and GFP-CNE-2 cells，and growth of GFP-CNE-2R tumors was not significantly inhibited by radiation. Conclusion GFP-CNE-2R xenograft tumors are more radioresistant than GFP-CNE-2 xenograft tumors.

Key words: Nasopharyngeal neoplasms, Xenograft tumor, Green fluorescent protein, Radiation resistance